Development of a Denaturing High-Performance Liquid Chromatography (DHPLC) Assay to Detect Parasite Infection in Grass Shrimp Palaemonetes pugio
نویسندگان
چکیده
منابع مشابه
Embryonic Development of the Grass Shrimp, Palaemonetes pugio, and the Influence of Salinity on Cardiac Physiology
...................................................................................................................................iii LIST OF FIGURES.......................................................................................................................viii CHAPTER
متن کاملDenaturing high performance liquid chromatography (DHPLC) used in the detection of germline and somatic mutations.
Denaturing high performance liquid chromatography (DHPLC) has been described recently as a method for screening DNA samples for single nucleotide polymorphisms and inherited mutations. Thirty-eight DNAs, 22 of which were heterozygous for previously characterized rearranged transforming gene (RET) or cystic fibrosis transmembrane conductance regulator gene (CFTR) mutations or polymorphisms, were...
متن کاملEffects of cyclic hypoxia on gene expression and reproduction in a grass shrimp, Palaemonetes pugio.
Cyclic changes in dissolved oxygen occur naturally in shallow estuarine systems, yet little is known about the adaptations and responses of estuarine organisms to cyclic hypoxia. Here we examine the responses of Palaemonetes pugio, a species of grass shrimp, to cyclic hypoxia (1.5-8 mg/l dissolved oxygen; 4.20-22.42 kPa) at both the molecular and organismal levels. We measured alterations in ge...
متن کاملThe role of native salinity regime on grass shrimp (Palaemonetes pugio) sensitivity to cadmium.
In euryhaline crustaceans, sensitivity to toxic trace metals may be linked to osmoregulation and salinity conditions. This study investigated if grass shrimp (Palaemonetes pugio) populations from different salinity regimes differed in sensitivity to cadmium (Cd). Grass shrimp were collected in May 2011 from two marsh sites with average salinities of ~3.0 ppt and 24.0 ppt. Groups were acclimated...
متن کاملDenaturing high-performance liquid chromatography: A review.
Denaturing high-performance liquid chromatography (DHPLC) compares two or more chromosomes as a mixture of denatured and reannealed PCR amplicons, revealing the presence of a mutation by the differential retention of homo- and heteroduplex DNA on reversed-phase chromatography supports under partial denaturation. Temperature determines sensitivity, and its optimum can be predicted by computation...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Fisheries and aquatic sciences
سال: 2012
ISSN: 2234-1749
DOI: 10.5657/fas.2012.0107